Transposase Enzyme-Linked Long-read Sequencing
One of the presently available commercial linked-read options. TELLseq data is very similar to 10X, except the
barcode is 18bp long and contained in the I1 read that Illumina provides with the standard R1 and R2 reads. The
barcode gets appended in the read header using a colon (:).
Specification
- barcode is the first 18bp of the I1 read
- barcode is appended to sequence header
- e.g.
@A00234534562:1:544:AATTATACCACAGCGGTA
- e.g.
- invalid barcode contains at least one
Ncharacter - advertised to have a capacity over 2 billion barcodes, but realistically use <24 million