10X

10X Genomics Linked Reads

The elder of the bunch and also a discontinued commercial product. 10X-style FASTQ files have the linked-read barcode as the first 16bp of the forward (R1) read. For these data to be compatible with the 10X LongRanger suite, the barcode must stay in the read. Moving the first 16bp into the read header breaks LongRanger compatibility.

Specification

• barcode is the first 16bp of the R1 read
• barcode stays in the sequence data for LongRanger compatibility
• limited to ~4.7 million barcodes