Single-Tuble Long Fragment Reads (stLFR)
Another of the presently available commercial linked-read options. stLFR data uses combinatorial barcodes
made up for three 10bp segments which are at the end of the R2 read. Demultiplexing these data results
in the barcode being moved to the sequence ID using a pound (#) sign between the sequence ID and barcode, with
the barcode recoded in the 1_2_3 format, where each segment is an integer.
Specification
- depending on the link sequence between segments, will be either the last 54bp or 42bp of the
R2read- 54 base barcode: 10+6+10+18+10
- 42 base barcode: 10+6+10+6+10
- barcode appended to sequence header with
#sign- e.g.
@A003432423434:1:324#12_432_1
- e.g.
- invalid barcode segment encoded as
0(e.g.,1_0_29) - advertised to have a capacity over 3.6 billion, with up to 50 million per sample (actual results may vary)